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| Registros recuperados: 47 | |
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| Trentin,A.G.; Alvarez-Silva,M.. |
| Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008 |
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| Minguell,J.J.; Conget,P.; Erices,A.. |
| Within the complex cellular arrangement found in the bone marrow stroma there exists a subset of nonhematopoietic cells referred to as mesenchymal progenitor cells (MPC). These cells can be expanded ex vivo and induced, either in vitro or in vivo, to terminally differentiate into at least seven types of cells: osteocytes, chondrocytes, adipocytes, tenocytes, myotubes, astrocytes and hematopoietic-supporting stroma. This broad multipotentiality, the feasibility to obtain MPC from bone marrow, cord and peripheral blood and their transplantability support the impact that the use of MPC will have in clinical settings. However, a number of fundamental questions about the cellular and molecular biology of MPC still need to be resolved before these cells can be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal progenitor cells; Transplantation; Differentiation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800003 |
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| Ferreira,L.R.; Velano,C.E.E.; Braga,E.C.; Paula,C.C.; Martéli Junior,H.; Sauk,J.J.. |
| Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (ER) membrane through a protein-conducting channel called translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as being essential for translocation of polypeptide chains into the cisterns of the ER. Sec61alpha forms a protein complex with collagen and Hsp47, an ER-resident heat shock protein that binds specifically to collagen. However, it is not known whether Sec61alpha is ubiquitously produced in collagen-producing F9 teratocarcinoma cells or under heat shock treatment. Furthermore, the production and utilization of Sec61alpha may depend on the stage of cell differentiation. Cultured F9 teratocarcinoma cells are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sec61alpha; Collagen type IV; Retinoic acid; F9 cells; Differentiation. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100005 |
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| FELMER,RICARDO N; CLARK,JOHN A. |
| The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a... |
| Tipo: Journal article |
Palavras-chave: Genetic ablation; NTR/CB1954; Adipocytes; Differentiation. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009 |
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| Wu, Chuan-fen; Delsert, Claude; Faure, Sandrine; Traverso, Edwin E.; Kloc, Malgorzata; Kuang, Jian; Etkin, Laurence D.; Morin, Nathalie. |
| Tumorhead (TH) regulates neural plate cell proliferation during Xenopus early development, and gain or loss of function prevents neural differentiation. TH shuttles between the nuclear and cytoplasmic/cortical cell compartments in embryonic cells. In this study, we show that subcellular distribution of TH is important for its functions. Targeting TH to the cell cortex/membrane potentiates a TH gain of function phenotype and results in neural plate expansion and inhibition of neuronal differentiation. We have found that TH subcellular localization is regulated, and that its shuttling between the nucleus and the cell cortex/cytoplasm is controlled by the catalytic activity of p21-activated kinase, X-PAK1. The phenotypes of embryos that lack, or have excess,... |
| Tipo: Text |
Palavras-chave: Actin cytoskeleton; Differentiation; Proliferation; Neural plate; P21 activated kinase; Tumorhead. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2996.pdf |
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| Alibardi,Lorenzo. |
| The present ultrastructural study on developing and regenerating feathers of chick and zebrafinch describes the ultrastructural changes that occur during the differentiation of barb cells that leads to the formation of the ramus of barbs. Differently from barbule and barb cortical cells that accumulate feather keratin, barb medullary cells undergo to lipid degeneration. Eventually, lipids disappear and medullary cells become empty cavities in the central part of the ramus. In barb medullary cells feather keratin is accumulated in few peripheral bundles that merge with those of cortical cells to fom the wall of the ramus. The latter is joined with branching barbules. The process that controls the transition from keratin-synthesizing to lipid-producing barb... |
| Tipo: Journal article |
Palavras-chave: Feathers; Barbs; Differentiation; Lipidization; Ultrastructure. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000100010 |
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| Barrena Figueroa, Ramo; Sanchez Garcia, Mercedes. |
| Due to a high level of product substitution in the food market, it often proves difficult to strike a balance between supply and demand. This is especially true in sectors with a such high level of competition and product differentiation as the wine producing sector. Faced with the difficulty of differentiating the product in terms of its technical characteristics, quality and price, therefore, a useful alternative is to explore what consumers perceive to be its "emotional benefits", since these have been shown to have a decisive effect on consumer purchasing decisions. For marketing purposes, an understanding of how consumers' personality traits condition their choice of products can help manufacturers to improve their strategic positioning in the market.... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Wine; Consumer behaviour; Laddering; Emotions; Differentiation; Demand and Price Analysis. |
| Ano: 2007 |
URL: http://purl.umn.edu/9435 |
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| Chambolle, Claire; Villas-Boas, Sofia Berto. |
| This paper shows that retailers may choose to offer products differentiated in quality to consumers, not to relax downstream competition, but to improve their buyer power in the negotiation with their supplier. We consider a simple vertical industry where two producers sell products differentiated in quality to two retailers who operate in separated markets. In the game, first retailers choose which product to carry, then each retailer and her chosen producer bargain over the terms of a two-part tariff contract and retailers finally choose the quantities. When upstream production costs are convex, the share of the total profits going to the retailer would be higher if they choose to differentiate. We thus isolate the wish to differentiate as "only" due to... |
| Tipo: Working or Discussion Paper |
Palavras-chave: Buyer Power; Product line; Differentiation; Marketing; L13; L42. |
| Ano: 2007 |
URL: http://purl.umn.edu/6866 |
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| Monterio, G.F.A.; Farina, Elizabeth Maria Mercier Querido; Nunes, R.. |
| The paper analyzes competition among supermarkets in Brazil. In contrast to part of the economic literature which suggests that the fast growth of big supermarket chains would destroy independent, medium and small supermarkets, the paper argues that big supermarket chains can coexist with different formats of independent food retailing. As a result, competition in food retail is complex and cannot be described as a simple Darwinian process of market concentration. The analysis is divided in two parts. In the first part, the competition between hypermarkets and supermarkets is examined. Evidences for the district of Sao Paulo, Brazil, suggest that these retailers form separate markets. The second part is focused on neighborhood supermarkets. The results... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Food retail; Supermarkets; Differentiation; Agribusiness; Industrial Organization. |
| Ano: 2008 |
URL: http://purl.umn.edu/44199 |
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| Schwindt,Telma T.; Motta,Fabiana L.; Gabriela F.,Barnabé; Cristina G.,Massant; Guimarães,Alessander O.; Calcagnotto,Maria Elisa; Pesquero,João B.; Mello,Luiz E.. |
| Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neural precursor cells; Neurosphere; Fibroblast growth factor 2; Epidermal growth factor; Differentiation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009 |
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| MA,CHANG M. |
| In higher vertebrates, from amphibians to humans, epidemial maturation is a conserved developmental process. Using adult epidemial tissue and an established keratinocyte cell line, the mouse Nkx-2.3 homeobox gene was demonstrated, for the first time, to be expressed in mouse epidermal keratinocytes. Under the normal culture condition, the spontaneous aggregation phenomenon, a common initiation step of ES cell differentiation, and the induction of mouse adult K1 keratin, a marker of mature epidermal keratinocytes, were both observed in vitro when the Xenopus Nkx-2.3 gene was stably transfected into a mouse pluripotent P19 EC cell line. The induction of mouse K1 keratin by using its Xenopus orthologous gene in the mouse P19 cell implies that Nkx-2.3 may play... |
| Tipo: Journal article |
Palavras-chave: Nkx-2.3; Epidermis; Homeobox gene; Skin maturation; Differentiation. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300001 |
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| Registros recuperados: 47 | |
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